To elucidate a whole-length cDNA sequence containing a canine G-CSF signal sequence and to provide a method for simply mass producing a canine G-CSF.

The method for producing a canine granulocyte colony stimulating factor comprises preparing an insect gene expression vector incorporated with a DNA encoding a canine granulocyte colony stimulating factor composed of a specific amino acid sequence, making a gene recombination baculovirus incorporated with the vector and producing a canine granulocyte colony stimulating factor using the gene recombination baculovirus.