Title:
METHOD OF PRODUCING CELL SHEET
Document Type and Number:
Japanese Patent JP2012065632
Kind Code:
A
Abstract:
To provide a method of efficiently producing a cell sheet in which the adhesion of cells is held.
The method of producing a cell sheet includes: a process which cultivates a cell on a substrate for cell culture containing an iron crosslinking alginic acid gel; and a process in which a chelating agent which specifically combines with iron (deferoxamine mesilate, deferasirox or deferiprone) is used, thereby the substrate for cell culture containing an iron crosslinking alginic acid gel is dissolved to obtain a cultivated cell as a cell sheet.
Inventors:
SANO IKUKO
NAMIKI HIDEO
NAMIKI HIDEO
Application Number:
JP2010215380A
Publication Date:
April 05, 2012
Filing Date:
September 27, 2010
Export Citation:
Assignee:
FRONTIER KK
International Classes:
C12N5/071; C12M3/04
Domestic Patent References:
| JP2009011215A | 2009-01-22 | |||
| JP2004135571A | 2004-05-13 | |||
| JP2007259776A | 2007-10-11 |
Attorney, Agent or Firm:
Hidekazu Miyoshi
Iwa Saki Kokuni
Masakazu Ito
Shunichi Takahashi
Toshio Takamatsu
Iwa Saki Kokuni
Masakazu Ito
Shunichi Takahashi
Toshio Takamatsu