Title:
培養細胞の製造方法，脊髄損傷疾患の治療剤の製造方法
Document Type and Number:
Japanese Patent JP7207742
Kind Code:
B2
Abstract:
[Problem to Be Solved] The present invention is to provide a method of culturing a cell, which method safely increases GDNF mRNA expression without introducing a gene with a virus.[Means to Solve the Problem] A method of producing a cultured cell comprising glial cell line-derived neurotrophic factor (GDNF) mRNA, comprising the step of culturing a human skin-derived stem cell in a serum-free medium comprising at least one of SAG, purmorphamine and sonic hedgehog (SHH) protein. The medium may further comprise B-27 supplement, a ROCK inhibitor, EGF and FGF2.
Inventors:
Hiroshi Nishina
Hiroko Yanaga
Hiroko Yanaga
Application Number:
JP2019551884A
Publication Date:
January 18, 2023
Filing Date:
August 02, 2018
Export Citation:
Assignee:
Regeneration Science Co., Ltd.
International Classes:
C12N15/01; A61K35/30; A61K35/36; A61P25/00; C12N5/10
Domestic Patent References:
| JP2002531128A | ||||
| JP2013502915A | ||||
| JP2005502377A | ||||
| JP2017163898A | ||||
| JP2007520207A |
Foreign References:
| WO2009148170A1 | ||||
| WO2011007900A1 |
Other References:
CHEN, J,K., et. al.,Small molecule modulation of Smoothened activity,Proceedings of the National Academy of Sciences of the United States of America,2002年10月29日,Vol. 99, NO. 22,p. 14071-14076
CHEN, W., et. al.,Activity-dependent internalization of smoothened mediated by beta-arrestin 2 and GRK2.,Science,2004年12月24日,Vol. 306,p. 2257-2260
CHEN, W., et. al.,Activity-dependent internalization of smoothened mediated by beta-arrestin 2 and GRK2.,Science,2004年12月24日,Vol. 306,p. 2257-2260
Attorney, Agent or Firm:
Hiroyuki Hirose
Daisuke Seki
Daisuke Seki
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