To provide a method for inexpensively and efficiently producing the subject enzyme through expressing it as a soluble protein in Escherichia coli.
This method for producing N-acetylglucosaminyltransferase (GnT) is characterized by comprising the following steps: first, Escherichia coli is transformed with an expression vector containing a chimeral DNA where a DNA encoding maltose-binding protein(MBP) and a DNA encoding GnT are mutually linked so as to express as a fused protein derived from MBP and GnT, the resultant transformant is then cultured to form the fused protein as a soluble protein, and the fused protein is purified from the cultured product by making use of the specific affinity of MBP, thus producing MBP-GnT fused protein; thereafter, the MBP fraction is eliminated from the above fused protein and GnT is collected.
SEKI TATSUJI
DANIEL G MORAN
FUJIYAMA KAZUHITO
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