Title:
リン脂質の製造方法
Document Type and Number:
Japanese Patent JP4978751
Kind Code:
B2
Abstract:
Provided is a method for producing a phospholipid at low cost by reusing phospholipase A2 in a method for producing the phospholipid whereby an arbitrary fatty acid is bonded to the 2-position of a phospholipid using an esterification reaction catalyzed by phospholipase A2 in glycerol. The method for producing a phospholipid is characterized by comprising conducting an esterification reaction catalyzed by phospholipase A2 between a lysophospholipid and an acyl donor in glycerol to from a phospholipid, adding a solvent immiscible with glycerol to form a glycerol layer and a solvent layer, extracting said phospholipid into said solvent layer, allowing phospholipase A2 to migrate into said glycerol layer, and, after separating the glycerol layer and distilling off the solvent remaining therein, further adding to the residual glycerol solution the lysophospholipid and the acyl donor to thereby conduct the esterification reaction again with use of phospholipase A2 remaining in said glycerol solution.
Inventors:
Tatsushi Tanaka
Yugo Iwasaki
Yugo Iwasaki
Application Number:
JP2011521910A
Publication Date:
July 18, 2012
Filing Date:
July 05, 2010
Export Citation:
Assignee:
Kaneka Corporation
Nagoya University
Nagoya University
International Classes:
C12P9/00
Domestic Patent References:
| JP2010068799A | 2010-04-02 | |||
| JPH05236974A | 1993-09-17 |
Attorney, Agent or Firm:
Takao Yanagino
Norio Morioka
Hisayoshi Sekiguchi
Masato Nakagawa
Norio Morioka
Hisayoshi Sekiguchi
Masato Nakagawa