バチルス細胞内でのポリペプチドの製法

Title:

バチルス細胞内でのポリペプチドの製法

Document Type and Number:

Japanese Patent JP4571304

Kind Code:

B2

Abstract:

The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a Bacillus host cell in a medium conducive for the production of the polypeptide, wherein the Bacillus cell comprises a nucleic acid construct comprising (i) a tandem promoter in which each promoter sequence of the tandem promoter is operably linked to a single copy of a nucleic acid sequence encoding the polypeptide and alternatively also (ii) an mRNA processing/stabilizing sequence located downstream of the tandem promoter and upstream of the nucleic acid sequence encoding the polypeptide; and (b) isolating the polypeptide from the cultivation medium. The present invention also relates to methods for producing a polypeptide, comprising: (a) cultivating a Bacillus host cell in a medium conducive for the production of the polypeptide, wherein the Bacillus cell comprises a nucleic acid construct comprising (i) a "consensus" promoter having the sequence TTGACA for the "-35" region and TATAAT for the "-10" region operably linked to a single copy of a nucleic acid sequence encoding the polypeptide and (ii) an mRNA processing/stabilizing sequence located downstream of the "consensus" promoter and upstream of the nucleic acid sequence encoding the polypeptide; and (b) isolating the polypeptide from the cultivation medium.

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Inventors:

William, Widner
Aroma, Sloma
Thomas, Michael Dee.

Application Number:

JP2000533574A

Publication Date:

October 27, 2010

Filing Date:

February 26, 1999

Export Citation:

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Assignee:

Novozymes, Incorporated

International Classes:

C12N15/09; C07K14/325; C12N1/21; C12N9/54; C12N15/32; C12N15/75; C12R1/07

Domestic Patent References:

JP62201583A
JP7504085A
JP8509609A

Other References:

HUNG,A.,MOLECULAR AND GENERAL GENETICS,1989年12月31日,V219 1989年発行,P129-136
Journal of Bacteriology,1995年,vol.177,p.3465-3471

Attorney, Agent or Firm:

Takashi Ishida
Jun Tsuruta
Kazuhiro Nakamura
Masaya Nishiyama
Higuchi Souji

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