Title:
バチルス細胞内でのポリペプチドの製法
Document Type and Number:
Japanese Patent JP5270498
Kind Code:
B2
Abstract:
The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a Bacillus host cell in a medium conducive for the production of the polypeptide, wherein the Bacillus cell comprises a nucleic acid construct comprising (i) a tandem promoter in which each promoter sequence of the tandem promoter is operably linked to a single copy of a nucleic acid sequence encoding the polypeptide and alternatively also (ii) an mRNA processing/stabilizing sequence located downstream of the tandem promoter and upstream of the nucleic acid sequence encoding the polypeptide; and (b) isolating the polypeptide from the cultivation medium. The present invention also relates to methods for producing a polypeptide, comprising: (a) cultivating a Bacillus host cell in a medium conducive for the production of the polypeptide, wherein the Bacillus cell comprises a nucleic acid construct comprising (i) a "consensus" promoter having the sequence TTGACA for the "-35" region and TATAAT for the "-10" region operably linked to a single copy of a nucleic acid sequence encoding the polypeptide and (ii) an mRNA processing/stabilizing sequence located downstream of the "consensus" promoter and upstream of the nucleic acid sequence encoding the polypeptide; and (b) isolating the polypeptide from the cultivation medium.
Inventors:
William, Widner
Aroma, Sloma
Thomas, Michael Dee.
Aroma, Sloma
Thomas, Michael Dee.
Application Number:
JP2009204651A
Publication Date:
August 21, 2013
Filing Date:
September 04, 2009
Export Citation:
Assignee:
Novozymes, Incorporated
International Classes:
C12N15/09; C07K14/325; C12N1/21; C12N9/54; C12N15/32; C12N15/75; C12R1/07
Foreign References:
| WO1994025612A1 |
Other References:
J. Bacteriol.,1995年,vol.177, no.12,pp.3465-3471
Mol. Microbiol.,1996年,vol.20, no.3,pp.633-643
Nucleic Acids Res.,1995年,vol.23, no.13,pp.2351-2360
Mol. Microbiol.,1996年,vol.20, no.3,pp.633-643
Nucleic Acids Res.,1995年,vol.23, no.13,pp.2351-2360
Attorney, Agent or Firm:
Atsushi Aoki
Takashi Ishida
Fukumoto product
Tetsuji Koga
Kazuhiro Nakamura
Yoichi Watanabe
Takashi Ishida
Fukumoto product
Tetsuji Koga
Kazuhiro Nakamura
Yoichi Watanabe