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Title:
METHOD OF PRODUCING VITAMIN B12 FROM HYDROGEN- ASSIMILATING METHANE BACTERIA
Document Type and Number:
Japanese Patent JP2001211894
Kind Code:
A
Abstract:

To produce vitamin B12 efficiently in a high content and a high recovery rate.

This method of producing vitamin B12 is provided by performing an accilimation of a middle temperature methane bacteria obtained from a digested sludge by using H2/CO2 as a medium, propagating the accilimated methane bacteria on a carrier including an inorganic nutritious salt of a small amount of a metal element by a fixed bed bioreactor, assimilating a mixed gas obtained by adding hydrogen to at least one kind of a coal gas and a biogas for reforming the mixture to methane, and at the same time, recovering cobalamine presenting in the fermented liquid as an extracellular product by using potassium cyanide as cyanocobalamine.


Inventors:
Maekawa, Takaaki
Cho, Shina
Application Number:
JP2000000024490
Publication Date:
August 07, 2001
Filing Date:
February 01, 2000
Export Citation:
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Assignee:
JAPAN SCIENCE & TECHNOLOGY CORP
International Classes:
C12M1/40; C12P3/00; C12P5/02; C12P19/42; (IPC1-7): C12P19/42; C12P3/00; C12P5/02
Domestic Patent References:
1992-01-09
1998-12-15
1990-11-26
1996-08-06
1997-09-09
1992-11-20