PURPOSE: To enable to prepare the subject peptide in high purity, yield and amount by dissolving a specific crude product in the aqueous solution of guanidine chloride or urea.
CONSTITUTION: A DNA comprising an amino acid sequence coding a portion (or all portions) of the amino acid sequence of adenylate kinase (or an analogue thereof) is combined with a DNA comprising an amino acid sequence of a peptide to be fused to prepare a manifestation vector (A). The component A is introduced into an microorganism or cell, which is cultured to manifest an adenylate kinase-fused peptide(AKP). The cultured microorganisms or cells are crushed and subsequently subjected to a solid-liquid separation process to recover a solid content comprising a crude product (B) containing AKP. The component B is dissolved in a 10-100mM tris hydrochloric acid buffer solution (pH: 7.5-8.5) containing 4-6M guanidine hydrochloride or 3-7M urea and cysteine, etc., to prepare a solution (C). The solution C is dialyzed with a collodion membrane, etc., to reduce the concentration of urea, etc., to 0.1-10M, and the deposited solid content is separated and removed. The remained solution is purified by a chromatography method, etc., to produce the adenylate kinase- fused peptide.
MISAWA SATORU
FURUYA HIDEYUKI
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