To provide a method for simply separating residual saccharide components and colored substances from molasses or blackstrap molasses and producing the colored substances in a good efficiency.

This method for purifying the colored substances from the molasses or blackstrap molasses comprises a process of preparing a diluted molasses liquid by diluting the molasses or blackstrap molasses with water, a process of adjusting the pH of the diluted molasses liquid to 1-3 by adding an acid thereto, a process of separating the colored substance from the diluted molasses liquid by bringing the pH-adjusted molasses-diluted liquid in contact with a hydrophobic chromatographic resin, to adsorb the colored substance in the diluted liquid onto the resin and a process of recovering a liquid of eluted colored substance from the resin through bringing an aqueous alkaline solution in contact with the hydrophobic chromatographic resin adsorbing the colored substance.