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Title:
METHOD FOR PURIFYING AND CONCENTRATING SPECIFIC CELL FROM MANY KINDS OF CELL UTILIZING FLUORESCENT COLORING MATTER SELECTIVELY TAKEN INTO THE SPECIFIC CELL
Document Type and Number:
Japanese Patent JP2012080819
Kind Code:
A
Abstract:

To provide a labor-saving method for damagelessly purifying and concentrating stem cells derived from animal (including humans) and ES cells, iPS cells, marrow stem cells and somatic stem cells from other cells.

The method comprises the following process: by combining such two means as to use, as stem cell's indicators, a cell-detective/isolating apparatus installed with a long-wavelength laser device and a highly safe long-wavelength fluorescent coloring matter having been used for liver examinations from old times, stem cells that have been very difficult to attain the present purpose are detected, isolated, purified and concentrated safely, in high selectivity and inexpensively in easy operability.


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Inventors:
SASAKI KATSUNORI
YOSHIE SUSUMU
ITO JUN
YOKOYAMA TADAYUKI
SHIRASAWA SAKIKO
FUJIMURA HIROSHI
TAKEDA KAZUO
Application Number:
JP2010229521A
Publication Date:
April 26, 2012
Filing Date:
October 12, 2010
Export Citation:
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Assignee:
ONCHIP BIOTECHNOLOGIES INC
International Classes:
C12N5/077; A61K35/12; A61K35/407; A61P1/16; A61P43/00; C12M1/00; G01N15/14
Domestic Patent References:
JP4358888B12009-11-04
JP2009011326A2009-01-22
JP2006081482A2006-03-30
JP2005524831A2005-08-18
Foreign References:
KR20100086372A2010-07-30
WO2009119369A12009-10-01
Other References:
金子順一ら, 臨床外科, vol. 65, no. 5, JPN6015000180, 20 May 2010 (2010-05-20), pages 642 - 649, ISSN: 0002978864
平野達ら, 日本レーザー医学会誌, vol. 28, no. 2, JPN6015000181, 2007, pages 122 - 128, ISSN: 0002978865
TAKEDA, K., ET AL., CYTOMETRY PART B (CLINICAL CYTOMETRY), vol. 76, JPN6015000182, 2009, pages 395 - 407, ISSN: 0002978866
Attorney, Agent or Firm:
Kenichi Morita
Kenjiro Yamaguchi



 
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