Title:
METHODS FOR PURIFICATION OF MESSENGER RNA
Document Type and Number:
Japanese Patent JP2022001056
Kind Code:
A
Abstract:
To provide methods for purification of messenger RNA.SOLUTION: The present invention provides, among other things, methods of purifying messenger RNA (mRNA) including the steps of subjecting an impure preparation comprising in vitro synthesized mRNA to a denaturing condition, and purifying the mRNA from the impure preparation from step (a) by tangential flow filtration, where the mRNA purified from step (b) is substantially free of prematurely aborted RNA sequences and/or enzyme reagents used in in vitro synthesis. In some embodiments, the step (a) comprises adding a protein denaturing agent to the impure preparation.SELECTED DRAWING: None
Inventors:
MICHAEL HEARTLEIN
FRANK DEROSA
ANUSHA DIAS
SHRIRANG KARVE
FRANK DEROSA
ANUSHA DIAS
SHRIRANG KARVE
Application Number:
JP2021143908A
Publication Date:
January 06, 2022
Filing Date:
September 03, 2021
Export Citation:
Assignee:
TRANSLATE BIO INC
International Classes:
C12N15/10; C07H21/02
Domestic Patent References:
| JP6586075B2 | 2019-10-02 | |||
| JP2005505305A | 2005-02-24 | |||
| JP2007515959A | 2007-06-21 | |||
| JP2000500028A | 2000-01-11 |
Foreign References:
| WO2012077080A1 | 2012-06-14 |
Attorney, Agent or Firm:
Hidesaku Yamamoto
Natsuki Morishita
Takatoshi Iida
Daisuke Ishikawa
Kensaku Yamamoto
Natsuki Morishita
Takatoshi Iida
Daisuke Ishikawa
Kensaku Yamamoto