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Title:
METHOD FOR RECOVERING MICROORGANISM FROM ACTIVATED SLUDGE USED FOR TREATING METAL-CONTAINING WASTEWATER AND METHOD FOR IDENTIFYING MICROORGANISM
Document Type and Number:
Japanese Patent JP2004129510
Kind Code:
A
Abstract:

To provide a simple method for separating/recovering a microorganism existing in activated sludge used for treating metal-containing wastewater, a method for detecting the microorganism with which a DNA sequence can be read without inhibiting an enzymic reaction such as a polymerase chain reaction (PCR) and a monitoring technique for the microorganism in the activated sludge used for treating the metal-containing wastewater.

The method for recovering the microorganism from the activated sludge used for treating the metal-containing wastewater is characterized as follows. The activated sludge used for treating the metal-containing wastewater is regulated to pH≥7 and ≤10 and metal ions in the activated sludge are converted into a metal hydroxide. The activated sludge is then allowed to stand for a prescribed time to precipitate the metal hydroxide. The resultant supernatant is further filtered through a membrane having ≤0.5 μm pore diameter to separate the microorganism in the activated sludge on the filter membrane. The method for identifying the microorganism in the activated sludge used for treating the metal-containing wastewater is characterized as follows. The DNA of the recovered microorganism is extracted and a specific region of the DNA is then amplified to identify the microorganism based on the DNA sequence of the resultant DNA product.


Inventors:
ITO KIMIO
KATO TOSHIRO
MIKI OSAMU
Application Number:
JP2002000294857
Publication Date:
April 30, 2004
Filing Date:
October 08, 2002
Export Citation:
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Assignee:
NIPPON STEEL CORP
International Classes:
G01N33/48; C02F3/12; C12N1/02; C12N15/09; C12Q1/04; C12Q1/68; G01N1/04; G01N1/10; G01N21/77; G01N21/78; G01N27/447; G01N33/18; G01N33/483; G01N33/53; (IPC1-7): C12N1/02; C02F3/12; C12Q1/04; C12Q1/68; G01N1/04; G01N1/10; G01N21/77; G01N21/78; G01N27/447; G01N33/18; G01N33/48; G01N33/483; G01N33/53
Attorney, Agent or Firm:
Ishida Kei
Jun Tsuruta
Masaya Nishiyama



 
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