Title:
METHOD FOR RECOVERING PROTEIN BY RELEASING UBIQUITIN FROM UBIQUITINATED PROTEIN
Document Type and Number:
Japanese Patent JP2005006637
Kind Code:
A
Abstract:
To provide a method for recovering a protein from a ubiquitinated protein by recovering the ubiquitinated protein with an immunological method such as an affinity chromatography or immuno-precipitation, etc., by using a specific antibody recognizing a ubiquinone chain and by releasing the ubiquitin from the ubiquitinated protein by using a de-ubiquitinating enzyme.
This method for recovering the protein which may be decomposable through a ubiquitin dependent protein decomposition pathway, by using an anti-ubiquitin chain antibody and the de-ubiquitinating enzyme.
Inventors:
KAYA MOTOHIRO
TAMURA NORIKO
TAKEUCHI JUNKO
TAMURA TOMOHIRO
TAMURA NORIKO
TAKEUCHI JUNKO
TAMURA TOMOHIRO
Application Number:
JP2003273076A
Publication Date:
January 13, 2005
Filing Date:
July 10, 2003
Export Citation:
Assignee:
NAT INST OF ADV IND & TECHNOL
International Classes:
G01N27/62; C07K1/22; C07K14/00; C07K16/18; C12N9/60; C12N15/09; C12P21/06; C12P21/08; G01N27/447; (IPC1-7): C12N15/09; C07K1/22; C07K16/18; C12N9/60; C12P21/06; G01N27/447; G01N27/62
Attorney, Agent or Firm:
Yusuke Hiraki
Satoshi Fujita
Natsuo Tanaka
Satoshi Fujita
Natsuo Tanaka