To provide a method and an apparatus for separating and concentrating a cell for regenerating a tissue according to simple operations in an incomplete opened system.

This method for separating the cell for regenerating the biotissue comprises capturing the cell for regenerating the tissue from a mixture liquid of the cell for regenerating the biotissue with impurity cells using a cell separating filter, then introducing a fluid into the cell separating filter and recovering the cell. The apparatus for separating the cell for regenerating the biotissue comprises a connecting means 1 for a raw material cell injecting tool connected to the upstream side of an inlet for the cell separating filter 3 and having a valve communicating only when connected to the raw material cell injecting tool, a connecting means 7 for a fluid injecting tool connected to the downstream side of an outlet for the cell separating filter 3 or to the upstream side of the inlet therefor and having a valve communicating only when connected to the fluid injecting tool for injecting the fluid into the cell separating filter 3 and a cell recovering means 6 connected through the cell separating filter 3 to the connecting means 7 for the fluid injecting tool on the mutual opposite side on the upstream side of the inlet for the cell separating filter 3 or the downstream side of the outlet therefor.