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Title:
METHOD FOR SUPPLYING OXYGEN AND CULTURE DEVICE USED THEREFOR
Document Type and Number:
Japanese Patent JPH06133765
Kind Code:
A
Abstract:

PURPOSE: To provide the method for performing the effective supply of oxygen by aerating a culture solution in the culture of vital cells even when the culture solution contains flocculated cell blocks and a microcarrier, and to provide the culture device.

CONSTITUTION: A stirrer 4 and a porous bulkhead 9 are disposed in a culture tank 1, and a culture solution 3 in which the cells of an organism are suspended is further received in the culture tank 1. The porous bulkhead 9 is connected to an oxygen- dissolving tank 2 disposed outside the culture tank through a piping 7 for circulating the oxygen-dissolved solution. Aeration nozzles 8a and 8b are disposed in the piping 7 at places near to its opening ports. The porous bulkhead 9 has fine holes for inhibiting the passage of cell blocks and a microcarrier and enables the contact mixing of the oxygen-dissolved solution with the culture solution through the fine holes of the bulkhead to supply the oxygen to the vital cells. The solution aeration method capable of supplying a large volume of oxygen can be applied even to a culture solution in which particulate solid substances such as the cell blocks and the microcarrier for the adhesion of the cells are suspended, and a high density culture can be performed.


Inventors:
HAGA RYOICHI
MURAKAMI HIKARI
NITSUTA MICHIYO
MATSUZAKI HARUMI
Application Number:
JP1992000292868
Publication Date:
May 17, 1994
Filing Date:
October 30, 1992
Export Citation:
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Assignee:
HITACHI LTD
International Classes:
C12M1/04; C12M3/00; C12N1/00; C12N5/00; C12N5/02; C12N5/04; (IPC1-7): C12N5/02; C12M1/04; C12M3/00; C12N1/00; C12N5/04; C12N5/06
Attorney, Agent or Firm:
平木 祐輔



 
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