Title:
METHOD OF SYNTHESIZING NUCLEIC ACID
Document Type and Number:
Japanese Patent JP2007236392
Kind Code:
A
Abstract:
To provide a method of synthesizing nucleic acid without complicated temperature control.
The present invention relates to an oligonucleotide having a novel structure and a method of synthesizing nucleic acid by using the same as a primer. This oligonucleotide is provided at the 5'-side of the primer with a nucleotide sequence substantially the same as a region synthesized with this primer as the origin of synthesis. The present invention realizes synthesis of nucleic acid based on an isothermal reaction with a simple constitution of reagents. Further, the present invention provides a method of synthesizing highly specific nucleic acid on the basis of this method of synthesizing nucleic acid.
COPYRIGHT: (C)2007,JPO&INPIT
Inventors:
NOUTOMI TSUGUNORI
HASE SATORU
HASE SATORU
Application Number:
JP2007113523A
Publication Date:
September 20, 2007
Filing Date:
April 23, 2007
Export Citation:
Assignee:
EIKEN CHEMICAL
International Classes:
C12N15/10; C12N15/09; C12Q1/68
Domestic Patent References:
| JPH11179056A | 1999-07-06 | |||
| JP2000037194A | 2000-02-08 | |||
| JPH07289298A | 1995-11-07 | |||
| JP4675141B2 | 2011-04-20 | |||
| JP2004141170A | 2004-05-20 |
Foreign References:
| US5874260A | 1999-02-23 | |||
| WO1996001327A1 | 1996-01-18 | |||
| WO1997004131A1 | 1997-02-06 |
Attorney, Agent or Firm:
Yusuke Hiraki
Ishii Teiji
Satoshi Fujita
Yuko Matsuniya
Ishii Teiji
Satoshi Fujita
Yuko Matsuniya