To find out the most efficient method in transformation method relating to targeted mutation induction in plant cells.
There is provided a method for targeted alteration of a double chain acceptor DNA sequence in a plant cell protoplast, which comprises combining the double chain acceptor DNA sequence with a donor mutagenic nucleobase, wherein the double chain acceptor DNA sequence contains a first DNA sequence and a second DNA sequence which is the complement of the first DNA sequence, wherein the donor mutagenic nucleobase comprises at least one mismatch with respect to the double chain acceptor DNA sequence to be altered, preferably with respect to the first DNA sequence. The method further comprises a step of introducing the donor mutagenic nucleobase into the cell protoplast using polyethylene glycol (PEG) mediated transformation and the use of PEG protoplast transformation for enhancing the rate of targeted mutagenesis.
DE BOTH MICHIEL THEODOOR JAN
LHUISSIER FRANCK
| JP2007167011A | 2007-07-05 |
| WO2007073166A1 | 2007-06-28 | |||
| WO2007073170A1 | 2007-06-28 | |||
| WO2007012138A1 | 2007-02-01 |
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Shuichi Fukuda
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