Title:
METHOD FOR TRANSFERRING EXTRANEOUS GENE INTO EARLY EMBRYO OF OVIPAROUS ANIMAL AND ELECTRODE TO BE USED THEREFOR
Document Type and Number:
Japanese Patent JP3631438
Kind Code:
B2
Abstract:
PROBLEM TO BE SOLVED: To provide a method for efficiently transferring an extraneous gene into the early embryo of an oviparous animal without affecting embryogenesis, and to provide electrodes to be used therefor.
SOLUTION: The method for transferring an extraneous gene into the early embryo of an oviparous animal by electroporation technique is characterized by involving perpendicularly applying electrical pulse to the blastodisc or blastoderm of the above early embryo injected with a DNA. The electrodes to be used for the above method are also provided.
Inventors:
Naito Mitsuru
Yasuhiko Hayakawa
Akiko Sano
Yasuhiko Hayakawa
Akiko Sano
Application Number:
JP2001046075A
Publication Date:
March 23, 2005
Filing Date:
February 22, 2001
Export Citation:
Assignee:
National Institute of Agriculture and Biological Sciences
Neppagene Co., Ltd.
Akiko Sano
Neppagene Co., Ltd.
Akiko Sano
International Classes:
C12M1/00; C12N5/10; C12N15/09; A01K67/027; (IPC1-7): C12N15/09; A01K67/027; C12M1/00; C12N5/10
Other References:
Jpn. Poult. Sci.,2000年,37,334-340
Attorney, Agent or Firm:
Fujiro Kubota
Yuya Yano
Yuya Yano
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