To provide a treatment method for biospecimen for fixing a reagent and a reaction product, while holding the position data of the target substrate in the biospecimen as pretreatment for analyzing the target substance for each individual cell in the biospecimen with satisfactory positional accuracy, and to provide an analysis method of the biospecimen.

In the reagent treatment method for biospecimen, a thin film constituted of a porous material is brought into contact with the biospecimen, containing cells or tissues, and a reagent solution is supplied to the specific region of a specimen by an ink jet method from the non-contact surface side of the thin film to perform supplying, holding and reacting of the reagent so as to be restricted to the region of a cell level within the surface of the biospecimen. Furthermore, in the analysis method of the biospecimen, the image data for the biospecimen are read and position data are recorded; and after the reagent treatment method is performed with respect to a specific region so as to be matched with the image data, the biospecimen is analyzed by microscopic observation or mass analysis.