PURPOSE: To enable the efficient recovery of a physiologically active substance from urine with good reproducibility on an industrial scale by adding a water-soluble organic medium and a specific metallic salt to the urine.
CONSTITUTION: Urine is preferably concentrated or treated with an adsorbent and the adsorbed fraction is eluted to provide a urine stock solution. A water-soluble organic medium, e.g. monohydric or polyhydric alcohols, aldehydes or ketones and at least one or more selected from metallic salts such as nitrates, sulfates, acetates or chlorides of Zn, Ca, Ba and Al are added to precipitate viscous substances present in the urine. The resultant precipitate is then removed with a centrifugal separator. A physiologically active substance such as a trypsin inhibitor or a colony stimulating factor is isolated from the obtained supernatant. Ethanol is preferred as the organic medium and added at a concentration within the range of 5-15vol.%. A Zn salt is preferred as the metallic salt and added at a concentration within the range of 0.1-1.0M.
SHIMIZU HIDEHARU
NIIMURA MORIO
SENOO KUNIKO
SUZUKI HIROYASU
