Title:
生物サンプル中のルシフェラーゼを検出するための方法及び組成物
Document Type and Number:
Japanese Patent JP4242560
Kind Code:
B2
Abstract:
The presence of renilla luciferase alone or both renilla luciferase and firefly luciferase is detected by adding reagent mixture(s) to a biological sample and producing glow luminescence having a duration of at least an hour. In producing luminescence from renilla luciferase alone, a reagent is added comprising coelenterazine, and dithiothreitol (DTT) and EDTA, or functional equivalents of DTT and EDTA. Luminescence from samples containing both firefly luciferase and renilla luciferase is produced by first adding a reagent comprising firefly luciferin, ATP, co-factors necessary for firefly luciferase activity (e.g., Ca+2 and Mg+2), dithiothreitol (DTT) or functional equivalents thereof, and AMP. Following measurement of the firefly luciferase, coelenterazine and EDTA, or functional equivalents of EDTA, are added, and the luminescence produced by renilla luciferase is measured.
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Inventors:
Rooland, Christiaan
Application Number:
JP2000529456A
Publication Date:
March 25, 2009
Filing Date:
January 18, 1999
Export Citation:
Assignee:
Packard Instrument Company Inc
International Classes:
C12Q1/66; G01N21/76; C12N9/99
Foreign References:
| WO1996040988A1 | ||||
| US5618682 |
Attorney, Agent or Firm:
Kazuo Shamoto
Shosuke Imai
Tadashi Masui
Yasushi Kobayashi
Hiroyuki Tomita
Takashi Nakata
Shosuke Imai
Tadashi Masui
Yasushi Kobayashi
Hiroyuki Tomita
Takashi Nakata