Title:
ＲＮＡ依存性標的ＤＮＡ修飾およびＲＮＡ依存性転写調節のための方法および組成物
Document Type and Number:
Japanese Patent JP6887479
Kind Code:
B2
Abstract:
The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms.
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Inventors:
Ginek, Martain
Champagne, emmanuel
Chirinsky, Kushiyutov
Daudna Kate, The James Harrison
Rim, wendel
Key, lei
Doudona, The Yenifar A
Champagne, emmanuel
Chirinsky, Kushiyutov
Daudna Kate, The James Harrison
Rim, wendel
Key, lei
Doudona, The Yenifar A
Application Number:
JP2019210828A
Publication Date:
June 16, 2021
Filing Date:
November 21, 2019
Export Citation:
Assignee:
The Regents of The University of California
University of Vienna
Champagne, emmanuel
University of Vienna
Champagne, emmanuel
International Classes:
C12N15/09; A61K31/7088; A61K38/46; A61K48/00; C07K19/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/16; C12N15/113; C12N15/55; C12N15/62; C12N15/63
Foreign References:
| US20100076057 |
Other References:
SAPRANAUSKAS, R. et al.,"The Streptococcus thermophilus CRISPR/Cas system provides immunity in Escherichia coli",Nucleic Acids Res.,2011年,Vol. 39,pp. 9275-9282
DELTCHEVA, E. et al.,"CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III",Nature,2011年,Vol. 471,pp. 602-607
GARNEAU J. E. et al.,"The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA",Nature,2010年,Vol. 468,pp. 67-71
PAPWORTH, Monika et al.,"Designer zinc-finger proteins and their applications",Gene,2006年,Vol. 366,pp. 27-38
DELTCHEVA, E. et al.,"CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III",Nature,2011年,Vol. 471,pp. 602-607
GARNEAU J. E. et al.,"The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA",Nature,2010年,Vol. 468,pp. 67-71
PAPWORTH, Monika et al.,"Designer zinc-finger proteins and their applications",Gene,2006年,Vol. 366,pp. 27-38
Attorney, Agent or Firm:
Makoto Ono
Kenkyo Kanayama
Mitsuaki Tsubokura
Kazuki Shigemori
Kenji Ando
Hidehiko Ichikawa
Yoshikazu Iwase
Yasufumi Shiroyama
Kenkyo Kanayama
Mitsuaki Tsubokura
Kazuki Shigemori
Kenji Ando
Hidehiko Ichikawa
Yoshikazu Iwase
Yasufumi Shiroyama