Title:
液体供給物の複数の成分を液体遠心クロマトグラフィーによって分離する方法および装置
Document Type and Number:
Japanese Patent JP4309914
Kind Code:
B2
Abstract:
A charge is injected at an intermediate point in a cell assembly and two phases alternate, with a first phase of duration t1 where the lighter solvent is injected at a first end of the device and a first component is received at the second end, and a second phase of duration t2 where the heavier solvent is injected at the second end and a second component is received at the first end : The charge in liquid solution has at least two components (A, B) of different partage coefficients such that they are entrained at unequal speeds respectively by a light solvent and a heavier solvent, in a device comprising at least one liquid-liquid centrifuge chromatography column (col) made by interconnection in series of at least one separation cell assembly (CE). The durations t1 and t2 and the rates of injection of the solvents are adjusted as a function of the components of the mixture, to obtain an optimal separation. A number of separations in cascade are used to isolate the components from each other in a mixture of more than two components. Two optical isomers are separated by injecting into a first device a charge containing the optical isomers and a chiral selector to obtain a first isomer and a mixture of the second isomer and the chiral selector. The mixture is then injected into a second device to separate the second isomer from the chiral selector. The charge can be injected continuously or discontinuously. An independent claim is made for a device to carry out the above process, which includes column(s) (col), a chain of separation cells (CE), a charge injection pump (P1), valves (V1, V2), receivers for the two separated components (FA, FB), and solvent injection pumps (P2, P3) for the lighter solvent (L) and the heavier solvent (l). The valves are alternated to change between the two phases. For mixtures with more than two components, at least two separation columns in cascade are used.
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Inventors:
Clardo, Francois
Fuco, Alan
Duran, Daniel
Fuco, Alan
Duran, Daniel
Application Number:
JP2006518263A
Publication Date:
August 05, 2009
Filing Date:
June 28, 2004
Export Citation:
Assignee:
ENI S.P.A.
Francois Clard
Francois Clard
International Classes:
G01N30/02; B01D15/08; G01N21/07; G01N30/26; G01N30/42; G01N30/88
Domestic Patent References:
| JP11183455A | ||||
| JP2179473A | ||||
| JP5281219A | ||||
| JP49079596A |
Other References:
J.L.den Holander, B.I.Stribos, M.J.van Buel, K.Ch.A.M.Luyben, and L.A.M.van der Wielen,Centrifugal partition chromatographic reaction for the production of chiral amino acids,Journal of Chromatography B,1998年 6月26日,Vol.711, No.1/2,P.223-235
Attorney, Agent or Firm:
Akio Miyazaki
Ishibashi Masayuki
Shinichi Iwata
Masaaki Ogata
Ishibashi Masayuki
Shinichi Iwata
Masaaki Ogata