Title:
ヒト多能性幹細胞由来の心筋細胞および心筋細胞サブポピュレーションの生成、同定ならびに単離のための方法
Document Type and Number:
Japanese Patent JP7089484
Kind Code:
B2
Abstract:
The present invention provides a method for generation, isolation, detection and/or analysis of cardiomyocytes derived from a starting cell composition comprising pluripotent and/or multipotent stem cells, the method comprising the steps a) differentiating said pluripotent and/or multipotent stem cells into cardiovascular cells, thereby generating a sample comprising cardiomyocytes and non-cardiomyocytes; and b) labeling the non-cardiomyocytes of said sample with more than one antibody or antigen binding fragment thereof specific for antigens of non-cardiomyocytes and c) depleting said labeled non-cardiomyocytes from said sample; and optionally d) labeling the cardiomyocytes of said sample with at least one antibody or antigen binding fragment thereof specific for antigen(s) of cardiomyocytes; and e) enriching said labeled cardiomyocytes and detecting and isolating cardiomyocyte subtypes derived from said pluripotent and/or multipotent stem cells.
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Inventors:
Noack, Christine
Ekkart, Dominic
Bosio, Andreas
Ekkart, Dominic
Bosio, Andreas
Application Number:
JP2018562924A
Publication Date:
June 22, 2022
Filing Date:
May 30, 2017
Export Citation:
Assignee:
Miltenii Biotech Bee Farr Untoko Carge
International Classes:
C12N5/077; A61K35/34; A61P9/00; C12Q1/02; G01N33/53; G01N33/543
Foreign References:
| WO2012135253A1 | ||||
| WO2014014119A1 |
Attorney, Agent or Firm:
Seiji Ohno
Yuko Matsutoya
Shinsuke Umeda
Yu Morita
Chisuke Konno
Nobuto Oki
Yuko Matsutoya
Shinsuke Umeda
Yu Morita
Chisuke Konno
Nobuto Oki