Title:
可溶性ＰＳＧＬ－１タンパク質変異体の精製のための方法
Document Type and Number:
Japanese Patent JP7065105
Kind Code:
B2
Abstract:
The present invention discloses a method for the purification of highly acidic recombinant proteins. The acidic protein is preferably the extracellular region of PSGL-1 or fusion and/or chimeric proteins comprising this soluble portion. Purification is a three-steps chromatography which comprises separation on an Anion Exchange solid-phase, a Hydrophobic Interaction and a Hydroxyapatite. Purity and yields are optimal and the process can be easily scaled up and automated.
Inventors:
Maisano Federico
Cliverin Federico
Cliverin Federico
Application Number:
JP2019542078A
Publication Date:
May 11, 2022
Filing Date:
February 06, 2018
Export Citation:
Assignee:
Bracco Suisse SA
International Classes:
C07K14/47; C07K1/16; C07K19/00
Domestic Patent References:
| JP2003528884A | ||||
| JP2016513643A | ||||
| JP2012507550A |
Foreign References:
| US5827817 | ||||
| WO2017137477A1 |
Attorney, Agent or Firm:
Masahiro Nanjo
Ayako Seta
Koichiro Inami
Hidemitsu Hara
Ayako Seta
Koichiro Inami
Hidemitsu Hara