Title:
ヒト胚性幹細胞の侵害受容器分化のための方法およびその使用
Document Type and Number:
Japanese Patent JP6868590
Kind Code:
B2
Abstract:
The present invention relates to the field of stem cell biology, in particular the linage specific differentiation of pluripotent or multipotent stem cells, which can include, but is not limited to, human embryonic stem cells (hESC), human induced pluripotent stem cells (hiPSC), somatic stem cells, cancer stem cells, or any other cell capable of lineage specific differentiation. Specifically described are methods to direct the lineage specific differentiation of hESC and/or hiPSC to nociceptors (i.e. nociceptor cells) using novel culture conditions. The nociceptors made using the methods of the present invention are further contemplated for various uses including, but limited to, use in in vitro drug discovery assays, pain research, and as a therapeutic to reverse disease of, or damage to, the peripheral nervous system (PNS). Further, compositions and methods are provided for producing melanocytes from human pluripotent stem cells for use in disease modeling.
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Inventors:
Lorenz Student
Stuart M. Chambers
Stuart M. Chambers
Application Number:
JP2018079947A
Publication Date:
May 12, 2021
Filing Date:
April 18, 2018
Export Citation:
Assignee:
Memorial Sloan Kettering Cancer Center
International Classes:
C12N5/074; C12N5/0735; C12N5/0793; C12N5/0797; C12N5/10; C12N15/09
Domestic Patent References:
| JP2013532961A |
Foreign References:
| WO2009099152A1 | ||||
| WO2007113505A1 |
Other References:
CHAMBERS,S.M. et al.,Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling.,Nat. Biotechnol.,2009年 3月,Vol.27, No.3,pp.275-80
CRAWFORD,T.Q. AND ROELINK,H.,The notch response inhibitor DAPT enhances neuronal differentiation in embryonic stem cell-derived e,Dev. Dyn.,2007年 3月,Vol.236, No.3,pp.886-92
YU,P.B. et al.,BMP type I receptor inhibition reduces heterotopic [corrected] ossification.,Nat. Med.,2008年12月,Vol.14, No.12,pp.1363-9
CRAWFORD,T.Q. AND ROELINK,H.,The notch response inhibitor DAPT enhances neuronal differentiation in embryonic stem cell-derived e,Dev. Dyn.,2007年 3月,Vol.236, No.3,pp.886-92
YU,P.B. et al.,BMP type I receptor inhibition reduces heterotopic [corrected] ossification.,Nat. Med.,2008年12月,Vol.14, No.12,pp.1363-9
Attorney, Agent or Firm:
Hidesaku Yamamoto
Natsuki Morishita
Natsuki Morishita