Title:
電気泳動ゲルから生体分子を単離し、かつ収集する方法、カセット、ゲル及び装置
Document Type and Number:
Japanese Patent JP2010502962
Kind Code:
A
Abstract:
Electrophoresis systems, assemblies, cassettes and methods for easily, and more effectively and efficiently, isolating a biomolecule band from an electrophoretic gel are provided. The methods use an electrophoresis cassette with at least one loading well and at least one collection well. A sample containing the biomolecule of interest is placed into at least one loading well and buffer or water is placed in at least one collection well. An electric field is then applied to drive migration and separation of the sample into different component bands within the gel. When the component of interest is located within at least one collection well, the electric field is terminated and the buffer or water in the collection well is removed, thereby isolating and collecting the sample component of interest.
Inventors:
Margarito Irana
Application Number:
JP2009526932A
Publication Date:
January 28, 2010
Filing Date:
August 31, 2007
Export Citation:
Assignee:
Life Technologies Corporation
International Classes:
G01N27/447; C07K1/26; C12N15/09; G01N27/62
Domestic Patent References:
| JPS63222254A | 1988-09-16 | |||
| JP2005241477A | 2005-09-08 | |||
| JP2004294317A | 2004-10-21 | |||
| JP2004155687A | 2004-06-03 | |||
| JP2004309326A | 2004-11-04 | |||
| JP2004290109A | 2004-10-21 |
Attorney, Agent or Firm:
Hatsushi Shimizu
Toshi Gobe
Koichi Niimi
Tomohiko Kobayashi
Shinichi Watanabe
Ryuichi Inoue
Masato Ozeki
Toshi Gobe
Koichi Niimi
Tomohiko Kobayashi
Shinichi Watanabe
Ryuichi Inoue
Masato Ozeki