Title:
METHODS AND COMPOSITIONS FOR DETECTING BACTERIAL CONTAMINATION
Document Type and Number:
Japanese Patent JP2019187431
Kind Code:
A
Abstract:
To provide compositions, reaction mixtures, kits and systems for detecting bacterial contamination.SOLUTION: Disclosed herein is a method for detecting bacterial contamination of a platelet sample with any of at least eight bacterial species, comprising: a step of subjecting the sample or a portion thereof to a nucleic acid amplification reaction under the conditions to generate amplicons of about 800 bases or less, where the reaction mixture comprises a single primer pair and a single detectable probe, the primer pair flanks the amplicon, the amplicon comprises a conserved sequence to which the detectable probe hybridizes, the conserved sequence is identical between the eight bacterial species; and a step of detecting the hybridization of the probe with the amplicon where the hybridization of the probe with the amplicon yields a detectable signal to indicate bacterial contamination of the platelet sample with any of the at least eight bacterial species.SELECTED DRAWING: None
Inventors:
JAMES JIAN QUAN WANG
CUI XIANGMIN
CHAN HIUNAM
CUI XIANGMIN
CHAN HIUNAM
Application Number:
JP2019114426A
Publication Date:
October 31, 2019
Filing Date:
June 20, 2019
Export Citation:
Assignee:
DCH MOLECULAR DIAGNOSTICS INC
International Classes:
C12N15/11; C12Q1/6813; C12Q1/6844; C12Q1/686; C12Q1/6862; C12Q1/689
Attorney, Agent or Firm:
Hidesaku Yamamoto
Natsuki Morishita
Takatoshi Iida
Daisuke Ishikawa
Kensaku Yamamoto
Natsuki Morishita
Takatoshi Iida
Daisuke Ishikawa
Kensaku Yamamoto