METHODS AND COMPOSITIONS FOR INCREASING SOMATIC CELL NUCLEAR TRANSFER (SCNT) EFFICIENCY BY REMOVING HISTONE H3-LYSINE TRIMETHYLATION - The Children's Medical Center Corporation

Title:

METHODS AND COMPOSITIONS FOR INCREASING SOMATIC CELL NUCLEAR TRANSFER (SCNT) EFFICIENCY BY REMOVING HISTONE H3-LYSINE TRIMETHYLATION

Document Type and Number:

Japanese Patent JP2023026679

Kind Code:

A

Abstract:

To provide methods and compositions for improving the efficiency of somatic cell nuclear transfer (SCNT), and the consequent production of nuclear transfer ESC (ntESC) and transgenic cells and/or transgenic non-human animals.SOLUTION: A method for increasing the efficiency of somatic cell nuclear transfer (SCNT) comprises contacting a donor mammalian cell, a recipient mammalian oocyte or a mammalian SCNT embryo with an agent which decreases H3K9 me3 methylation in the donor mammalian cell, recipient mammalian oocyte or mammalian SCNT embryo, thereby increasing the efficiency of the SCNT.SELECTED DRAWING: Figure 7

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Inventors:

Chang Yi
Shogo Matoba

Application Number:

JP2023001284A

Publication Date:

February 24, 2023

Filing Date:

January 06, 2023

Export Citation:

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Assignee:

The Children's Medical Center Corporation

International Classes:

C12N15/873; A01K67/027; C12N5/073; C12N5/0735; C12N5/10; C12N15/113

Attorney, Agent or Firm:

Shimizu Hatsushi
Masao Haruna
Hirotaka Yamaguchi
Shun Jinbe
Ryuichi Inoue
Toshimitsu Sato
Koichi Niimi
Tomohiko Kobayashi
Hideki Kodera
Masato Ozeki
Kazuya Kawamoto

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