Title:
METHODS AND COMPOSITIONS FOR NUCLEASE-MEDIATED TARGETED INTEGRATION OF TRANSGENES
Document Type and Number:
Japanese Patent JP2019088321
Kind Code:
A
Abstract:
To provide methods and compositions for homology-independent targeted insertion of donor molecules into the genome of a cell.SOLUTION: In one aspect, a double-stranded polynucleotide is provided comprising: an exogenous nucleic acid sequence; and a sequence comprising one or more target sites for one or more nucleases, where the one or more target sites are not within the exogenous nucleic acid sequence; where the double-stranded polynucleotide does not comprise homology arms. In another aspect, the exogenous nucleic acid sequence is at least 1 kb in length.SELECTED DRAWING: None
Inventors:
GREGORY J COST
FYODOR URNOV
WILLIAM M AINLEY
JOSEPH F PETOLINO
JAYAKUMAR PON SAMUEL
STEVEN R WEBB
LAKSHMI SASTRY-DENT
FYODOR URNOV
WILLIAM M AINLEY
JOSEPH F PETOLINO
JAYAKUMAR PON SAMUEL
STEVEN R WEBB
LAKSHMI SASTRY-DENT
Application Number:
JP2019026476A
Publication Date:
June 13, 2019
Filing Date:
February 18, 2019
Export Citation:
Assignee:
SANGAMO BIOSCIENCES INC
DOW AGROSCIENCES LLC
DOW AGROSCIENCES LLC
International Classes:
C12N15/09; A01H5/00; A01K67/027; C12N5/10; C12N15/11; C12N15/12; C12N15/29
Attorney, Agent or Firm:
Hidesaku Yamamoto
Natsuki Morishita
Takatoshi Iida
Daisuke Ishikawa
Kensaku Yamamoto
Natsuki Morishita
Takatoshi Iida
Daisuke Ishikawa
Kensaku Yamamoto
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