Title:
METHODS FOR DETERMINING ZYGOSITY OF FAD2 GENE IN CANOLA USING ENDPOINT PCR
Document Type and Number:
Japanese Patent JP2018068306
Kind Code:
A
Abstract:
PROBLEM TO BE SOLVED: To provide, in part, endpoint PCR assays for the detection and high throughput zygosity analysis of the fad-2 gene in canola.SOLUTION: Disclosed herein is a method for determining the zygosity of a canola plant having fad-2 gene, comprising a step in which a genomic DNA sample is taken from a canola plant; a step in which an amplicon is formed; a step in which a fluorescent probe preferentially forms a hybrid with the amplicon; and a step of showing that the sample contains a first allele with a single nucleotide polymorphism of interest and a second allele without the single nucleotide polymorphism of interest. Further disclosed is a related kit for determining zygosity from a sample of a canola plant or seed which can be used to uniquely identify the zygosity and variety of canola lines comprising the subject gene.SELECTED DRAWING: None
Inventors:
LASANTHA CHANDANA UBAYASENA
ZOE EHLERT
CHANDRA SHEKARA A CHANNABASAVARADHYA
ZOE EHLERT
CHANDRA SHEKARA A CHANNABASAVARADHYA
Application Number:
JP2017234075A
Publication Date:
May 10, 2018
Filing Date:
December 06, 2017
Export Citation:
Assignee:
DOW AGROSCIENCES LLC
International Classes:
C12Q1/68; A01H5/00; A01H6/20; C12Q1/6809; C12Q1/6827; C12Q1/686; C12Q1/6895
Attorney, Agent or Firm:
Hiroshi Kobayashi
Norio Omori
Nao Fujita
Yasuhito Suzuki
Norio Omori
Nao Fujita
Yasuhito Suzuki
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