Title:
3MHz~125MHzのパルス無線周波数を組み込む波形を用いるエレクトロポレーションによる細菌形質転換のための方法および装置
Document Type and Number:
Japanese Patent JP2007535943
Kind Code:
A
Abstract:
A method and apparatus for electroporation includes placing a mixture of bacterial suspension and transforming DNA into an electroporation cuvette. The resulting sample is subjected through a current-limiting device to a complex 5 waveform including a burst of high-voltage radio-frequency current, which in some embodiments is superimposed on a biphasic high-voltage DC pulse, and in other embodiments on a high-voltage lower-frequency AC burst. The total waveform has at least an initial portion greater than eleven thousand volts per centimeter of electrode spacing, and a later portion in some embodiments is reduced to less than thirty percent 10 of magnitude of the initial portion. Transformed bacteria are selected by culture in selective medium in an embodiment. The high-voltage radio-frequency current is between 3 and 125 MHz, and in an embodiment is 24 MHz.
Inventors:
Thulin, Michal Buoy.
Lind, Lee Earl.
Sullivan, Charles Earl.
Lind, Lee Earl.
Sullivan, Charles Earl.
Application Number:
JP2007511709A
Publication Date:
December 13, 2007
Filing Date:
May 06, 2005
Export Citation:
Assignee:
The Trustees of Dartmouth College
International Classes:
C12N15/09; C12M1/00; C12M1/42; C12N13/00; C12N15/00; A61N1/32
Other References:
JPN6009066664, Ｊ． Ａｐｐｌ． Ｍｉｃｒｏｂｉｏｌ．, 2000, ｖｏｌ．８， ｎｏ．２, ｐ．２２０−２２７
JPN6009066666, Ａｐｐｌ． Ｅｎｖｉｒｏｎ． Ｍｉｃｒｏｂｉｏｌ．, 2004, ｖｏｌ．７０， ｎｏ．２, ｐ．８８３−８９０
JPN6009066667, Ｂｉｏｅｌｅｃｔｒｏｃｈｅｍｉｓｔｒｙ, 2000, ｖｏｌ．５１， ｎｏ．２, ｐ．１３３−１４３
JPN6009066666, Ａｐｐｌ． Ｅｎｖｉｒｏｎ． Ｍｉｃｒｏｂｉｏｌ．, 2004, ｖｏｌ．７０， ｎｏ．２, ｐ．８８３−８９０
JPN6009066667, Ｂｉｏｅｌｅｃｔｒｏｃｈｅｍｉｓｔｒｙ, 2000, ｖｏｌ．５１， ｎｏ．２, ｐ．１３３−１４３
Attorney, Agent or Firm:
Hidesaku Yamamoto
Takaaki Yasumura
Natsuki Morishita
Takaaki Yasumura
Natsuki Morishita