Title:
プログラマブル・アレイ顕微鏡を用いた光学共焦点結像のための方法および装置
Document Type and Number:
Japanese Patent JP7053845
Kind Code:
B2
Abstract:
Optical confocal imaging, being conducted with a programmable array microscope (PAM) (100), having a light source device (10), a spatial light modulator device (20) with a plurality of reflecting modulator elements, a PAM objective lens and a camera device (30), wherein the spatial light modulator device (20) is configured such that first groups of modulator elements (21) are selectable for directing excitation light to conjugate locations of an object to be investigated and for directing detection light originating from these locations to the camera device (30), and second groups of modulator elements (22) are selectable for directing detection light from non-conjugate locations of the object to the camera device (30), comprises the steps of directing excitation light from the light source device (10) via the first groups of modulator elements to the object to be investigated, wherein the spatial light modulator device (20) is controlled such that a predetermined pattern sequence of illumination spots is focused to the conjugate locations of the object, wherein each illumination spot is created by at least one single modulator element defining a current PAM illumination aperture, collecting image data of a conjugate image lc, based on collecting detection light from conjugate locations of the object for each pattern of PAM illumination apertures, collecting image data of a non-conjugate image lnc, based on collecting detection light from non-conjugate locations of the object for each pattern of PAM illumination apertures via the second groups of modulator elements (22) with a non-conjugate camera channel of the camera device (30), and creating an optical sectional image of the object (OSI) based on the image data of the conjugate image lc and the non-conjugate image lnc, wherein the step of collecting the image data of the conjugate image lc includes collecting a part of the detection light from the conjugate locations of the object for each pattern of PAM illumination apertures via modulator elements of the second groups of modulator elements (22) surrounding the current PAM illumination apertures with the non-conjugate camera channel of the camera device (30). Furthermore, a PAM calibration method and PAMs being configured for the above methods are described.
Inventors:
Jobin, Thomas M
Defreeze, Antony Harvey
Arnd-Jovin, Donna Yacht
Defreeze, Antony Harvey
Arnd-Jovin, Donna Yacht
Application Number:
JP2020534865A
Publication Date:
April 12, 2022
Filing Date:
December 20, 2017
Export Citation:
Assignee:
MAX-PLANCK-GESELLSCHAFT ZUR FOeRDERUNG DER WISSENSCHAFTEN E.V.
International Classes:
G02B21/00; G01N21/64; G02B26/08
Domestic Patent References:
| JP11194275A | ||||
| JP2013522684A | ||||
| JP2006317544A | ||||
| JP2003517638A | ||||
| JP11249023A | ||||
| JP2011100012A | ||||
| JP2015513671A |
Foreign References:
| US20160202178 |
Other References:
DE VRIES ANTHONY H B; ET AL,GENERATION 3 PROGRAMMABLE ARRAY MICROSCOPE (PAM) FOR HIGH SPEED LARGE FORMAT 以下備考,PROCEEDINGS OF SPIE,SPIE,2015年03月10日,VOL:9376,,PAGE(S):93760C1 - 15,http://dx.doi.org/10.1117/12.2076390,OPTICAL SECTIONING IN FLUORESCENCE
Attorney, Agent or Firm:
Mitsuharu Kawakami
Kawamoto Manabu
Kawamoto Manabu