Title:
METHODS OF IDENTIFYING BACTERIA COMPRISING BINDING POLYPEPTIDES
Document Type and Number:
Japanese Patent JP2022104923
Kind Code:
A
Abstract:
To provide: methods of identifying bacteria comprising binding polypeptides; methods of identifying bacteria with improved expression of binding polypeptides; methods of identifying binding polypeptides with improved expression; engineered bacteria suitable for use in the methods of the invention; compositions that are obtained using the methods, for example, anti-IL-13 antibodies with improved expression and/or stability; and libraries comprising binding polypeptides.SOLUTION: Provided is a method of identifying a bacterium comprising a binding polypeptide that specifically binds to a target molecule, the method comprising the steps of: (a) providing a bacterium expressing a nucleic acid encoding a candidate binding polypeptide; (b) subjecting the bacterium to conditions that permeabilize the outer membrane of the bacterium; (c) contacting the bacterium with a detectably labeled target molecule; (d) resealing the outer membrane of the bacterium following step (c); and (e) identifying the bacterium as comprising a binding polypeptide by the presence of the labeled target molecule.SELECTED DRAWING: None
Inventors:
TWYLA NOELLE LOMBANA
REBEKAH MCKENNA
CHRISTOPH SPIESS
MICHAEL DILLON
KARTHIK VEERAVALLI
REBEKAH MCKENNA
CHRISTOPH SPIESS
MICHAEL DILLON
KARTHIK VEERAVALLI
Application Number:
JP2022042055A
Publication Date:
July 12, 2022
Filing Date:
March 17, 2022
Export Citation:
Assignee:
GENENTECH INC
International Classes:
C12Q1/04; A61K39/395; A61P1/04; A61P11/00; A61P11/02; A61P11/06; A61P17/00; A61P19/10; A61P27/02; A61P31/14; A61P37/08; A61P43/00; C07K16/00; C07K16/46; C07K19/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N15/13; C12N15/62; C12N15/63; C12P21/08; C40B40/08
Attorney, Agent or Firm:
Sonoda/Kobayashi Patent Business Corporation