Title:
変異を導入するための方法
Document Type and Number:
Japanese Patent JP7413283
Kind Code:
B2
Abstract:
A method for introducing mutations into at least one target nucleic acid molecule comprises: (a) providing at least one sample comprising at least one target nucleic acid molecule; and (b) introducing mutations into the at least one target nucleic acid molecule by amplifying the at least one target nucleic acid molecule using a DNA polymerase to provide a mutated at least one target nucleic acid molecule, wherein step (b) is carried out using dNTPs at unequal concentrations and in the presence of a nucleotide analog.
Inventors:
Monaghan, Ray G
To, Joyce
Burke, Katherine M.
Immelfort, Michael
Darling, Aaron Yee
To, Joyce
Burke, Katherine M.
Immelfort, Michael
Darling, Aaron Yee
Application Number:
JP2020566338A
Publication Date:
January 15, 2024
Filing Date:
February 19, 2019
Export Citation:
Assignee:
ILLUMINA SINGAPORE PTE LTD
International Classes:
C12Q1/6844; C12N15/10; C12N15/11; C12P21/00; C12Q1/6869
Foreign References:
| WO2011106368A2 |
Other References:
Methods in Molecular Biology,2002年,Vol.192,pp.167-174
NUCLEOSIDES & NUCLEOTIDES,1997年,Vol.16,pp.1749-1752
Frontiers in Microbiology,2014年,Vol.5,Article224
Nucleic Acids Research,2016年,Vol.44,pp.1022-1035
Nucleic Acids Reserach,2010年,Vol.38,pp.8095-8104
Molecules,2010年,Vol.15,pp.8229-8240
NUCLEOSIDES & NUCLEOTIDES,1997年,Vol.16,pp.1749-1752
Frontiers in Microbiology,2014年,Vol.5,Article224
Nucleic Acids Research,2016年,Vol.44,pp.1022-1035
Nucleic Acids Reserach,2010年,Vol.38,pp.8095-8104
Molecules,2010年,Vol.15,pp.8229-8240
Attorney, Agent or Firm:
Patent Attorney Corporation Hiraki International Patent Office
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