Title:
メチル化されたＤＮＡの標的領域に基づいてシーケンシングライブラリーを構築する方法及びキット
Document Type and Number:
Japanese Patent JP7203276
Kind Code:
B2
Abstract:
Disclosed are a method and system for constructing sequencing library based on target region of methylated DNA, and uses thereof. The method includes: obtaining a transformed DNA sample with a universal sequence; performing amplification using a first specific primer located upstream of the target region and a first universal primer at least partially matching or overlapping the universal sequence; and performing amplification using a second specific primer, a second universal primer and a tagged primer to obtain the sequencing library. The second specific primer is located downstream of the first specific primer and upstream of the target region, the second universal primer overlaps at least a partial sequence of the second specific primer, and the tagged primer overlaps a partial sequence of the first universal primer. Alternatively, the second specific primer is located downstream of the target region, the second universal primer overlaps at least a partial sequence of the first specific primer, and the tagged primer overlaps a partial sequence of the second specific primer.
Inventors:
Yanglin
張艷艷
King Sowei
Rohka
Chen Fang
Jiang Hui
張艷艷
King Sowei
Rohka
Chen Fang
Jiang Hui
Application Number:
JP2022502317A
Publication Date:
January 12, 2023
Filing Date:
May 21, 2019
Export Citation:
Assignee:
MGI TECH CO., LTD.
International Classes:
C12Q1/6869; C12Q1/6853; C12Q1/6876
Foreign References:
| US20150011396 | ||||
| WO2018103025A1 | ||||
| US20190002872 |
Attorney, Agent or Firm:
Hideki Miyata
Hiroaki Sakai
Hiroaki Sakai