Title:
METHODS OF PRODUCING HOMOGENEOUS PLASTIC-ADHERENT APTAMER-MAGNETIC BEAD-FLUOROPHORE AND OTHER SANDWICH ASSAYS
Document Type and Number:
Japanese Patent JP2014131512
Kind Code:
A
Abstract:
PROBLEM TO BE SOLVED: To provide an assay format which enables rapid, one-step (homogeneous) assays for a variety of analytes without wash steps and without influencing sensitivity.SOLUTION: The present invention provides methods of using assembly of DNA aptamer-magnetic bead (MB) conjugate, aptamer-quantum dot (QD), aptamer-fluorescent nanoparticle or other aptamer-fluorescent molecule, aptamer-chemiluminescent reporter, aptamer-radioisotope, or other aptamer-reporter sandwich assays that enable adherence to glass, polystyrene and other plastics. Adherence to glass or plastics enables fluorescence from fluorescent molecules partitioned and concentrated on a surface to be detected from background (bulk solution) fluorescence in one step (without a wash step) even after an external magnetic field for enhancing the assay efficiency is removed.
Inventors:
BRUNO JOHN G
Application Number:
JP2014025743A
Publication Date:
July 17, 2014
Filing Date:
February 13, 2014
Export Citation:
Assignee:
OTC BIOTECHNOLOGIES LLC
International Classes:
C12N15/09; C12Q1/68; G01N33/569
Domestic Patent References:
| JP2006511819A | 2006-04-06 | |||
| JP2003531379A | 2003-10-21 | |||
| JP2002529705A | 2002-09-10 |
Foreign References:
| WO2005049826A1 | 2005-06-02 |
Attorney, Agent or Firm:
Taro Yaguchi