Title:
リボ毒性の一時的な減少に基づき、細胞毒性組換えポリペプチドをスクリーニングし、選択し、同定する方法
Document Type and Number:
Japanese Patent JP6814634
Kind Code:
B2
Abstract:
The present invention relates to methods of screening libraries of chimeric molecules comprising ribotoxic polypeptides, where screening is based on the interim reduction or elimination of ribotoxicity and the methods can identify cytotoxic molecules, each comprising a binding region and a ribotoxic region which jointly possess a desired assay-selectable characteristic, such as, e.g., binding to a target biomolecule, binding to a target cell, and/or cellular internalization.
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Inventors:
Poma Eric
Willat Erin
Kim jason
Higgins Jack
Willat Erin
Kim jason
Higgins Jack
Application Number:
JP2016548156A
Publication Date:
January 20, 2021
Filing Date:
February 04, 2015
Export Citation:
Assignee:
MOLECULAR TEMPLATES, INC.
International Classes:
C12N15/62; C07K19/00; C12P21/02; C40B30/04; G01N33/15; G01N33/50
Domestic Patent References:
| JP2011050388A | ||||
| JP2009530468A | ||||
| JP2007536905A | ||||
| JP2002503453A |
Foreign References:
| WO2012154530A1 | ||||
| WO2009032954A1 | ||||
| WO2014164680A1 |
Other References:
The Journal of Biological Chemistry,2011年,Vol.286, No.6,p.4165-4172
Molecular Microbiology,1998年,Vol.29, No.4,p.1019-1027
Toxicon,2011年,Vol.57,p.525-539
Molecular Microbiology,1998年,Vol.29, No.4,p.1019-1027
Toxicon,2011年,Vol.57,p.525-539
Attorney, Agent or Firm:
Masaki Hirota
Seiji Ozawa
Yusaku Tokai
Kazuhiro Matsuda
Makoto Horiuchi
Masako Yamauchi
Shuichi Sonomoto
Akihiro Yamamura
Satoshi Morikawa
Hiroyuki Tomita
Seiji Ozawa
Yusaku Tokai
Kazuhiro Matsuda
Makoto Horiuchi
Masako Yamauchi
Shuichi Sonomoto
Akihiro Yamamura
Satoshi Morikawa
Hiroyuki Tomita