Title:
幹細胞培養のためのマイクロキャリア
Document Type and Number:
Japanese Patent JP6943937
Kind Code:
B2
Abstract:
We disclose a particle comprising a matrix coated thereon and having a positive charge, the particle being of a size to allow aggregation of primate or human stem cells attached thereto. The particle may comprise a substantially elongate, cylindrical or rod shaped particle having a longest dimension of between 50µm and 400µm, such as about 200µm. It may have a cross sectional dimension of between 20µm and 30µm. The particle may comprise a substantially compact or spherical shaped particle having a size of between about 20µm and about 120µm, for example about 65µm. We also disclose a method of propagating primate or human stem cells, the method comprising: providing first and second primate or human stem cells attached to first and second respective particles, allowing the first primate or human stem cell to contact the second primate or human stem cell to form an aggregate of cells and culturing the aggregate to propagate the primate or human stem cells for at least one passage. A method of propagating human embryonic stem cells (hESCs) in long term suspension culture using microcarriers coated in Matrigel or hyaluronic acid is also disclosed. We also disclose a method for differentiating stem cells.
Inventors:
Oh, Steve
Lesina, marti
Chu, Andre
Louveny shawl
Zweigelt, Robert
Chen, Allen
Lesina, marti
Chu, Andre
Louveny shawl
Zweigelt, Robert
Chen, Allen
Application Number:
JP2019211253A
Publication Date:
October 06, 2021
Filing Date:
November 22, 2019
Export Citation:
Assignee:
AGENCY FOR SCIENCE,TECHNOLOGY AND RESEARCH
International Classes:
C12N11/02; C12N5/0735; C12N5/0789; C12N11/08; C12N11/12
Foreign References:
| US20070264713 | ||||
| US20050147959 |
Attorney, Agent or Firm:
Shinjiro Ono
Osamu Yamamoto
Toru Miyamae
Motoharu Nakanishi
Osamu Yamamoto
Toru Miyamae
Motoharu Nakanishi