Title:
ミスマッチエンドヌクレアーゼ及び使用方法
Document Type and Number:
Japanese Patent JP4395632
Kind Code:
B2
Abstract:
We describe here an in vitro method of increasing complementarity in a heteroduplex polynucleotide sequence. The method uses annealing of opposite strands to form a polynucleotide duplex with mismatches. The heteroduplex polynucleotide is combined with an effective amount of enzymes having strand cleavage activity, 3' to 5' exonuclease activity, and polymerase activity, and allowing sufficient time for the percentage of complementarity to be increased within the heteroduplex. Not all heteroduplex polynucleotides will necessarily have all mismatches resolved to complementarity. The resulting polynucleotide is optionally ligated. Several variant polynucleotides result. At sites where either of the opposite strands has templated recoding in the other strand, the resulting percent complementarity of the heteroduplex polynucleotide sequence is increased. Also described are mismatch endonucleases suitable for use in the process.
Inventors:
Paget, Haru Es.
Beefongs, Andrew A.
Bodjani, Fakley S.
Smith, Mark El.
Lindbow, John A.
Fitz Morris, Wayne P.
Beefongs, Andrew A.
Bodjani, Fakley S.
Smith, Mark El.
Lindbow, John A.
Fitz Morris, Wayne P.
Application Number:
JP2003566160A
Publication Date:
January 13, 2010
Filing Date:
January 31, 2003
Export Citation:
Assignee:
Novi Biotech, Limited Liability Company
International Classes:
C12N15/09; C07K14/08; C12N9/16; C12N9/22; C12N15/10; C12N15/82; C12P21/00; C12Q1/34; C12Q1/68
Domestic Patent References:
| JP2000511774A |
Foreign References:
| WO2001062974A1 | ||||
| WO1999029902A1 |
Other References:
SOLARO, P. C. et al.,"Endonuclease VII of phage T4 triggers mismatch correction in vitro.",J. MOL. BIOL.,1993年,Vol.230,p.868-877
OLEYKOWSKI, C. A. et al.,"Mutation detection using a novel plant endonuclease.",NUCLEIC ACIDS RESEARCH,1998年,Vol.26, No.20,p.4597-4602
OLEYKOWSKI, C. A. et al.,"Mutation detection using a novel plant endonuclease.",NUCLEIC ACIDS RESEARCH,1998年,Vol.26, No.20,p.4597-4602
Attorney, Agent or Firm:
Atsushi Aoki
Takashi Ishida
Fukumoto product
Tetsuji Koga
Yoichi Watanabe
Kazuhiro Nakamura
Masaru Nakajima
Takashi Ishida
Fukumoto product
Tetsuji Koga
Yoichi Watanabe
Kazuhiro Nakamura
Masaru Nakajima