Title:
MODIFIED GLUTAMIC ACID DECARBOXYLASE
Document Type and Number:
Japanese Patent JP2023027835
Kind Code:
A
Abstract:
To provide a GAD having higher GABA productivity and properties (thermal stability, pH, productivity as an enzyme) suitable for production on an industrial scale, and a method for producing GABA which includes using the GAD, and a method for producing GABA which includes activating a host cell into which the GAD gene is introduced. The present invention provides a polypeptide that includes an amino acid sequence at least 85% identical to an amino acid sequence described in a sequence number 1 or 2, and has glutamic acid decarboxylase activity. Further, the present invention provides an enzyme agent for producing γ-amino butyric acid (GABA) including the polypeptide, and a method for producing GABA which includes using the enzyme agent for producing the GABA. Further, the present invention provides a polynucleotide encoding the polypeptide, a vector including the polynucleotide, a host cell transformed by the vector, and a method for producing GABA which includes activating the host cell.SELECTED DRAWING: None
Inventors:
ITO SOHEI
NAKANO SHOGO
TAKAGI HIROSHI
KOZUKA KOHEI
MIMURA KENTA
IIZUKA TAKAHISA
NAKANO SHOGO
TAKAGI HIROSHI
KOZUKA KOHEI
MIMURA KENTA
IIZUKA TAKAHISA
Application Number:
JP2021133138A
Publication Date:
March 03, 2023
Filing Date:
August 18, 2021
Export Citation:
Assignee:
SHIZUOKAKEN KORITSU DAIGAKU HOJIN
JAPAN MAIZE PROD
JAPAN MAIZE PROD
International Classes:
C12N15/60; C12N1/19; C12N1/21; C12N9/88; C12N15/31; C12N15/63; C12P13/00
Attorney, Agent or Firm:
Sykes Patent Attorney Corporation