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Title:
MODIFIED HEAT-RESISTANT DNA POLYMERASE
Document Type and Number:
Japanese Patent JP2014079236
Kind Code:
A
Abstract:

To provide a modified DNA polymerase belonging to family B, not being inhibited by dUTP.

The modified DNA polymerase has modifications of at least two amino acids in an amino acid sequence relating to binding of uracil and lower uracil sensitivity than a wild-type DNA polymerase.


Inventors:
KOBAYASHI TETSUDAI
Application Number:
JP2013072516A
Publication Date:
May 08, 2014
Filing Date:
March 29, 2013
Export Citation:
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Assignee:
TOYO BOSEKI
International Classes:
C12N15/09; C12N9/12; C12Q1/68
Domestic Patent References:
JP2005526510A2005-09-08
JP2006507012A2006-03-02
JP2002253265A2002-09-10
JPH1042872A1998-02-17
JP2010505410A2010-02-25
JP2010035533A2010-02-18
JP2007504817A2007-03-08
JP2003144143A2003-05-20
JP2018000610A2018-01-11
JP2013072515A2013-04-22
JP2013072517A2013-04-22
Other References:
PROTEIN ENG. DES. SEL., vol. 23, no. 8, JPN6013054808, 2010, pages 589 - 597, ISSN: 0003473631