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Title:
MODIFIED HEAT-STABLE DNA POLYMERASE
Document Type and Number:
Japanese Patent JP2014079235
Kind Code:
A
Abstract:

To provide a modified family B DNA polymerase not inhibited by dUTP.

Provided is a modified DNA polymerase that has at least one amino acid modification in one of the amino acid sequences participating in uracil binding, and at least one amino acid modification in one of the amino acid sequences corresponding to 3'-5' exonuclease activity regions. The polymerase has a lower sensitivity to uracil than the wild type polymerase.


Inventors:
KOBAYASHI TETSUDAI
Application Number:
JP2013072515A
Publication Date:
May 08, 2014
Filing Date:
March 29, 2013
Export Citation:
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Assignee:
TOYO BOSEKI
International Classes:
C12N15/09; C12N9/12; C12Q1/68
Domestic Patent References:
JP2006507012A2006-03-02
JPH1042872A1998-02-17
JP2002253265A2002-09-10
JP2005526510A2005-09-08
JP2010505410A2010-02-25
JP2010035533A2010-02-18
JP2007504817A2007-03-08
JP2003144143A2003-05-20
JP2013072516A2013-04-22
JP2013072517A2013-04-22
JP2017093924A2017-06-01
Other References:
PROTEIN ENG. DES. SEL., vol. 23, no. 8, JPN6013054808, 2010, pages 589 - 597, ISSN: 0003473625