Title:
改変されたインスリンペプチドおよびその合成方法
Document Type and Number:
Japanese Patent JP4221392
Kind Code:
B2
Abstract:
A method for protecting a peptide from peptidase activity in vivo, the peptide being composed of between 2 and 50 amino acids and having a C-terminus and an N-terminus and a C-terminus amino acid and an N-terminus amino acid is described. In the first step of the method, the peptide is modified by attaching a reactive group to the C-terminus amino acid, to the N-terminus amino acid, or to an amino acid located between the N-terminus and the C-terminus, such that the modified peptide is capable of forming a covalent bond in vivo with a reactive functionality on a blood component. In the next step, a covalent bond is formed between the reactive group and a reactive functionality on a blood component to form a peptide-blood component conjugate, thereby protecting said peptide from peptidase activity. The final step of the method involves the analyzing of the stability of the peptide-blood component conjugate to assess the protection of the peptide from peptidase activity.
Inventors:
Dominic P. Brydon
Alan Em. Ezrin
Peter G. Milner
Darren El. Holmes
Karen Cibodieu
Alan Em. Ezrin
Peter G. Milner
Darren El. Holmes
Karen Cibodieu
Application Number:
JP2005140407A
Publication Date:
February 12, 2009
Filing Date:
May 12, 2005
Export Citation:
Assignee:
Konjuchem Biotechnology, Inc.
International Classes:
A61K47/42; C07K14/645; A61K38/00; A61K38/02; A61K47/48; A61P1/18; A61P5/00; A61P25/00; A61P25/28; A61P43/00; C07K1/107; C07K1/113; C07K1/14; C07K2/00; C07K7/00; C07K14/00; C07K14/16; C07K14/465; C07K14/575; C07K14/605
Domestic Patent References:
| JP3178998A | ||||
| JP8510255A | ||||
| JP56158747A | ||||
| JP3502691A | ||||
| JP6025300A |
Foreign References:
| WO1995010302A1 | ||||
| WO1986005494A1 | ||||
| WO1998023643A1 |
Other References:
Nucleic Acids Research,1980年,Vol.8, No.18,pp.4091-4109
Arch. Pharm. Pharm. Med. Chem.,1998年,Vol.331, No.2,pp.47-53
Biol. Pharm. Bull.,1998年,Vol.21, No.1,pp.56-61
Life Sciences,1987年,Vol.41,pp.1243-1248
Eur. J. Biochem.,1980年,Vol.112,pp.383-388
Biochem. Biophys. Res. Commun.,1989年,Vol.160,pp.862-867
FEBS Letter,1987年,Vol.223, No.1,pp.74-76
Arch. Pharm. Pharm. Med. Chem.,1998年,Vol.331, No.2,pp.47-53
Biol. Pharm. Bull.,1998年,Vol.21, No.1,pp.56-61
Life Sciences,1987年,Vol.41,pp.1243-1248
Eur. J. Biochem.,1980年,Vol.112,pp.383-388
Biochem. Biophys. Res. Commun.,1989年,Vol.160,pp.862-867
FEBS Letter,1987年,Vol.223, No.1,pp.74-76
Attorney, Agent or Firm:
Hidesaku Yamamoto
Takaaki Yasumura
Natsuki Morishita
Takaaki Yasumura
Natsuki Morishita