Title:
MODIFIED MULLERIAN INHIBITING SUBSTANCE (MIS) PROTEINS AND USES THEREOF FOR TREATMENT OF DISEASES
Document Type and Number:
Japanese Patent JP2022180637
Kind Code:
A
Abstract:
To provide modified recombinant human Mullerian Inhibiting Substance (MIS) proteins which have improved cleavage, increased bioactivity, and increased potency, as compared to wild-type human MIS proteins.SOLUTION: Provided is a recombinant MIS protein comprising a combination of a modification of a non-MIS leader sequence or a functional fragment thereof in place of the MIS leader sequence in a particular amino acid sequence and a modification of at least one amino acid in a particular amino acid sequence, to increase cleavage as compared to in the absence of a modification, the recombinant MIS protein having increased cleavage and increased yield of production in vitro as compared to wild-type MIS protein.SELECTED DRAWING: Figure 1B
Inventors:
PATRICIA K DONAHOE
DEMETRIOS VAVVAS
DAVID PEPIN
HOANG MIEN VAN
DEMETRIOS VAVVAS
DAVID PEPIN
HOANG MIEN VAN
Application Number:
JP2022161002A
Publication Date:
December 06, 2022
Filing Date:
October 05, 2022
Export Citation:
Assignee:
MASSACHUSETTS GEN HOSPITAL
MASSACHUSETTS EYE & EAR INFIRMARY
MASSACHUSETTS EYE & EAR INFIRMARY
International Classes:
A61K31/337; C12N15/12; A61K31/416; A61K31/436; A61K31/704; A61K33/243; A61K38/22; A61K45/00; A61K47/64; A61P3/10; A61P5/28; A61P13/08; A61P15/00; A61P15/08; A61P17/00; A61P17/10; A61P17/14; A61P35/00; A61P43/00; C07K14/495; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N15/13; C12N15/14; C12N15/56; C12N15/62; C12N15/70; C12N15/861; C12N15/863; C12N15/867; C12P21/02
Attorney, Agent or Firm:
Shimizu Hatsushi
Masao Haruna
Hirotaka Yamaguchi
Shun Jinbe
Ryuichi Inoue
Toshimitsu Sato
Koichi Niimi
Tomohiko Kobayashi
Masato Ozeki
Kazuya Kawamoto
Masao Haruna
Hirotaka Yamaguchi
Shun Jinbe
Ryuichi Inoue
Toshimitsu Sato
Koichi Niimi
Tomohiko Kobayashi
Masato Ozeki
Kazuya Kawamoto