Title:
改変パラミクソウイルスおよびその作製方法
Document Type and Number:
Japanese Patent JP5102630
Kind Code:
B2
Abstract:
The present invention provides a modified paramyxovirus containing a reduced amount of receptor-binding protein compared with the wild type; a method of preparing a modified paramyxovirus, comprising the following steps: (1) a step for introducing a nucleic acid that suppresses the expression of a receptor-binding protein of a paramyxovirus into an animal cell, (2) a step for infecting the paramyxovirus to the cell, and (3) a step for isolating paramyxovirus particles replicated in the cell; and a modified paramyxovirus prepared by the method of preparation mentioned above. The present invention also provides a chimera protein wherein a fusion protein of a virus has been joined or bound to a peptide that binds specifically to a cell surface marker; a nucleic acid that encodes the chimera protein; an animal cell capable of expressing the chimera protein on the cell surface thereof; a modified paramyxovirus expressing the chimera protein on the virus particle surface thereof; and a method of preparing a tissue targeting paramyxovirus, comprising: (1) a step for supplying a nucleic acid that encodes a chimera protein wherein a fusion protein of a virus has been joined or bound to a peptide that binds specifically to a cell surface marker of the target cells, (2) a step for introducing the nucleic acid supplied in (1) into an animal cell in an expressible state, and expressing the same, (3) a step for infecting a paramyxovirus to the cell, and (4) a step for isolating paramyxovirus particles replicated in the cell.
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Inventors:
Yasufumi Kaneda
Katai Tamai
Kotaro Saga
Kawachi Masako
Katai Tamai
Kotaro Saga
Kawachi Masako
Application Number:
JP2007546543A
Publication Date:
December 19, 2012
Filing Date:
November 24, 2006
Export Citation:
Assignee:
Genomidia Co., Ltd.
International Classes:
C12N15/09; C07K14/115; C07K16/18; C07K19/00; C12N5/10; C12N7/00
Domestic Patent References:
| JP2004329222A | 2004-11-25 | |||
| JP2001522871A | 2001-11-20 | |||
| JPH08508648A | 1996-09-17 | |||
| JP2002514892A | 2002-05-21 | |||
| JP2001286282A | 2001-10-16 |
Other References:
JPN6012013452; Ｊｏｕｒｎａｌ ｏｆ Ｖｉｒｏｌｏｇｙ ｖｏｌ．７９, 200508, ｐ．１０４６７-１０４７７
JPN6012013453; Ｖｉｒｕｓ Ｒｅｓｅａｒｃｈ ｖｏｌ．１０２, 2004, ｐ．２７-３５
JPN6012013453; Ｖｉｒｕｓ Ｒｅｓｅａｒｃｈ ｖｏｌ．１０２, 2004, ｐ．２７-３５
Attorney, Agent or Firm:
Takashima Ichi
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