Title:
単一ストレスによる目的の遺伝子の持続された活性化を達成するための分子調節回路
Document Type and Number:
Japanese Patent JP4344092
Kind Code:
B2
Abstract:
The exposure of cells, tissues and organs to "stress," such as elevated temperature, stimulates production of active heat stress transcription factors (HSF), which in turn, induce expression of genes regulated by stress promoters. Normally, the activity of stress promoters declines after cells, tissues and organs are returned to a normal condition. Mutant forms of HSF, however, can constitutively transactivate stress genes, in the absence of stress. By taking advantage of such mutant HSF, molecular circuits can be devised to provide a sustained expression of a gene of interest using a single application of stress. One form of molecular circuit comprises (a) a first nucleic acid molecule that comprises a gene encoding a transcription factor and a promoter activatable by stress and by the transcription factor, wherein the stress-activatable promoter and the transcription factor gene are operably linked, and (b) a second nucleic acid molecule that comprises a gene of interest and a second promoter activatable by the transcription factor, wherein the second promoter and the gene of interest are operably linked.
Inventors:
Richard Volmee
Application Number:
JP2000547244A
Publication Date:
October 14, 2009
Filing Date:
May 04, 1999
Export Citation:
Assignee:
HSF PHARMACEUTICALS SA
International Classes:
C12N15/09; A61K35/76; A61K48/00; A61P7/00; A61P7/04; A61P9/00; A61P25/28; A61P31/00; A61P35/00; A61P37/08; A61P43/00; C07K14/435; C07K14/465; C07K14/47; C12N1/19; C12N5/02; C12N5/10; C12N7/00; C12N15/12; C12N15/63; C12N15/85; C12P21/02; C12Q1/68; C12Q1/6897
Domestic Patent References:
| JP2500801A |
Foreign References:
| WO1997035992A1 |
Other References:
Crit.Rev.Eukaryot.Gene Expr.,Vol.4,No.4(1994)p.357-401
Attorney, Agent or Firm:
Aoyama Aoi
Kyousei Tamura
Minato Saito
Kyousei Tamura
Minato Saito