Title:
MONOCLONAL ANTIBODY BOND DETERMINANT FACTOR OF GRAM NEGATIVEBACTERIA
Document Type and Number:
Japanese Patent JPS62228295
Kind Code:
A
Abstract:
The present invention provides novel hybridoma cell lines which produce monoclonal antibodies - (MoAbs) that bind epitopes found on lipopolysaccharide most commonly associated with the endotoxin core of gram negative bacteria and exhibit broad cross-reactivity with gram negative bacteria of different genera and effectively neutralize endotoxin. At least one of the MoAbs disclosed (XMMEN-J5D) binds an epitope also found on gram positive bacteria. The hybridomas are produced by fusing an immortal cell, a cell having the ability to replicate indefinitely in myeloma cell culture, and an effector immune cell following immunization of the immune cell host with a preparation of a gram negative bacteria. While several individual hybridoma cell lines producing monoclonal antibodies to lipopolysaccharide are described, the present invention adds to the state of the art an entire family of hybridomas producing monoclonal antibodies to lipopolysaccharide-associated epitopes. The monoclonal antibodies produced by the hybridoma cell lines the present invention are useful in the detection of bacterial infections, therapy and prophylaxis of bacterial endotoxemia and infection caused by gram negative bacteria.
Inventors:
ROOUERU ESU YANGU
Application Number:
JP22948186A
Publication Date:
October 07, 1987
Filing Date:
September 27, 1986
Export Citation:
Assignee:
UNIV CALIFORNIA
International Classes:
A61K39/02; C07K14/005; C07K14/195; C07K14/41; C07K16/00; C07K16/12; C12N15/02; C07K19/00; C12N5/00; C12N5/10; C12N15/00; C12N15/12; C12P21/00; C12P21/08; G01N33/569; G01N33/577; A61K38/00; C12R1/91; (IPC1-7): A61K39/02; C07K15/04; C12N5/00; C12N15/00; C12P21/00; C12R1/91; G01N33/577
Domestic Patent References:
| JPS60501242A | 1985-08-08 |
Foreign References:
| WO1985001659A1 | 1985-04-25 |
Attorney, Agent or Firm:
Kyozo Yuasa