PURPOSE: The titled cellular surface antigen recognition type antibody, produced by a hybridoma prepared by using a cellular strain (MKN-45) derived from a gastric low differentiation type glandular cancer as an immunization source, having a light chain of IgG2a and capable of exhibiting positive reaction to cancer cells, e.g. lung cancer, colonic cancer, gastric cancer, etc.
CONSTITUTION: A monoclonal antibody obtained by fractionating a cell extract of an established cancer cell (MKN-45) derived from a gastric low differentiation type glandular cancer by gel filtration, mixing the resultant high-molecular fraction having 700,000W1,500,000 molecular weight with complete Freund's adjuvant, immunizing an animal, e.g. mouse, with the mixture, collecting a cell producing an antibody, carrying out cell fusion with a myelomatous cell of an animal of the same species, cloning the resultant cell to give a cloned hybridoma, transplanting the resultant cloned hybridoma to the abdominal cavity of an animal, e.g. BALB/C mouse, to produce the aimed antibody capable of exhibiting the positive reaction to cancer cells of lung cancer (PC-3), colonic cancer (Colo201), gastric cancer (MKN-45, or KATO-III), hepatic cancer (HEK) and nasopharyngeal cancer (KB) and recovering and purifying the antibody from the ascites.
KAMIMURA YATSUHIRO
FUKUYAMA KAZUMI
KOBAYASHI TAKASHI
KANO YOSHIAKI
YAMANA RYUTARO
KASHIWAGI EIJI
NAGURA KAZUAKI
WATANABE MASAHIRO
NISHIDA MASAYUKI
SUYAMA TADAKAZU
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